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Membranes were blocked with 5% (w/v) milk powder in PBS for 1 h and incubated for 16 h at 4 C with one of the following primary antibodies: rabbit anti-SLC31A1/CTR1 (Abcam, Cambridge, UK), mouse anti-GAPDH (Santa Cruz Biotechnology, Dallas, TX, USA), mouse anti-XPF (Thermo Fisher, Waltham, MA, USA), mouse anti-ERCC1 (Santa Cruz Biotechnology), mouse anti-Tubulin (Abcam), rabbit anti-NRF2 (Abcam)
Proc Natl Acad Sci USA, 91:89558959 Glazebrook J, Rogers EE, Ausubel FM (1996) Isolation of Arabidopsis mutants with enhanced disease susceptibility by direct screening
We will then want to inject the appropriate site and the response to the injection will hopefully help prove the hypothesis
How NR supports NAD pathways After administration, NR follows the bodys natural NAD salvage pathway: Efficient cellular uptake: NR can be transported into cells
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