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Site-directed mutagenesis Mutant forms of mito GCL refractory to the shRNA against GCL ( mito GCLmut) or catalytically inactive mito GCL ( mito GCL (E103A)) were generated using the site-directed mutagenesis QuikChange XL kit (Stratagene, La Jolla, CA, USA), followed by Dpn I digestion
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Reduction of serum carnitine concentrations during anticonvulsant therapy with phenobarbital, valproic acid, phenytoin, and carbamazepine in children
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The results were remarkable: patients receiving the Cordyceps extract showed massive, statistically significant improvements in overall symptom severity compared to the control group, with profound reductions in fatigue scores, insomnia, and respiratory distress
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