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Cell culture and treatments The murine macrophage cell line RAW 264.7 (TIB-71, American Type Culture Collection, Manassas, VA, USA) was cultured at 37C and under 5% CO 2 condition in Dulbeccos modified Eagles medium (DMEM) containing 4.5 g/L glucose, 10% fetal bovine serum (FBS), and 1% penicillinstreptomycin (P/S) mixture

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Ipamorelin, a selective ghrelin receptor agonist, works by binding to growth hormone secretagogue receptors in the pituitary gland, triggering targeted GH release without affecting cortisol or prolactin levels

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