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The structure also revealed an unexpected feature of EEFSec selectivity: a Sec-binding pocket lined by conserved residues (Thr242, Phe273, and Arg285) discriminates against canonical aminoacyl-tRNAs, while paradoxically accommodating Ser-tRNA [Ser]Sec raising the possibility that serine misincorporation at UGA codons may occur in vivo under selenium-limiting conditions

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These diverse and interconnected functions underscore the critical importance of mitochondria in maintaining cellular health and homeostasis, and perturbations in these processes have been linked to a wide range of diseases, including cardiovascular disorders and cognitive impairments, highlighting the need for further research into mitochondrial function and dysfunction