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Induction of shear stress by exposure to fluid movement decreased apoptosis, enhanced proliferation, and the mobility of endothelial cells in these models but did not alter the expression of TJ proteins or P-gp (DeStefano et al., in vivo levels after ~12 days, and integrity is maintained over 3 weeks in culture

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While the precise mechanism underlying this hierarchical chemoattractant-induced PTEN redistribution remains unclear, it is likely to contribute to reduced PIP3 levels at the leading edge, thereby promoting neutrophil migration toward PI3K-independent chemoattractants