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Researchers have documented that each freeze-thaw cycle damages peptide chains, and pre-splitting eliminates the cumulative loss that comes from thawing one bulk vial multiple times
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RT-PCR amplification was performed using BlasTaq 2X qPCR MasterMix (ABMgood, Richmond, Canada), incorporating the cDNA template, gene-specific primers (Integrated DNA Technologies, Coralville, USA) (Table 1), and nuclease-free water (Vivantis Technologies, Shah Alam, Malaysia) in a final volume of 20 L
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