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Western blotting Total protein extracts were obtained after cell lysis in 20 mM MOPS (pH 6.5), 1% Triton X-100 (v/w) previously supplemented with 1 mM Na 3 VO 4 , 1 mM NaF, and 1 protease inhibitors (11836170001, Roche)
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It can be done at any time of day but try to be consistent with the time each week
The stone walls of the Walled City had gone, but the refugees were convinced the diplomatic ones remained
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