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Measurement of glutathione and glutathione disulfide [PMID: 23045054] Laboratory literature summary: Measurement of glutathione and glutathione disulfide
Instead of promoting GSH-conjugation reactions, cysteine-containing GSTs (Cys-GSTs) are able to perform deglutathionylation reactions similarly to glutaredoxins but the targets are usually different since glutaredoxin substrates are mostly oxidized proteins and Cys-GST substrates are metabolites
The most common are pneumonia, abscesses of the skin, tissues, and organs, suppurative arthritis (invasion of the joints by infectious agent leading to generation of pus), and osteomyelitis (infection of the bone)
Thus, we intercalated four FM copies (FM 4 ) between Nt 7 and GFP and performed the RD assay in HeLa cells as their larger cell bodies grant for a better visualization