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After washing in ultrapure water, the sections were heated at 60 C, air dried, and incubated in xylene for 5 min

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These include the POMC-derived peptides released by neighbouring keratinocytes and the neuropeptide calcitonin gene-related peptide, substance P, and neuropeptide Y released from intramucosal free nerve endings, which are in close contact with melanocytes in the basal cell layer of the epithelium