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refers to DNA methylation in the promoter region of GSTP1 gene responsible for epigenetic silencing of this isoenzyme
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Immunoblotting Cells were washed with PBS and lysed with lysis buffer (150 mM NaCl, 20 mM EDTA, 50 mM Tris-HCl, 0.5% NP-40) containing protease and phosphatase inhibitors
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