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Calculator presets covered by this guide Vial size 2 mg BAC water 1 mL Target research amount 300 mcg Vial size 5 mg BAC water 2 mL Target research amount 500 mcg Vial size 10 mg BAC water 3 mL Target research amount 300 or 500 mcg 01 Check the vial amount
For viability, 7-aminoactinomycin D (7AAD) (Thermo fisher A1310) or Zombie NIR (BioLegend 423106) diluted 1:3000 was stained concurrently with antibodies in all flow cytometry assays
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