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In this assay, the 30 min incubation duration was chosen to be optimal because of the inherently cytotoxic nature of CuCl 2 and Cu(OAc) 2 towards HaCaT keratinocytes, as they were observed to cause substantial cell death at a duration longer than 30 mins
The medication also influences the gut microbiome, which plays a crucial role in systemic inflammation levels
Therefore, obtaining ethical approval or undergoing ethics committee evaluation was not necessary for this work
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Several preclinical models are examining how this peptide can support processes such as cell repair and tissue remodeling
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