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Afterwards, cells were blocked, permeabilized and incubated with a primary mouse monoclonal antibody to H2AX (Ser-139) (Upstate Biotechnology, Lake Placid, NY, USA) diluted 1:500 for 16 h at 4 C, followed by incubation with anti-mouse FITC secondary antibody (Sigma-Aldrich) diluted 1:200 for 2 h at room temperature, or with a mouse anti-active caspase 3 (BD, Pharmigen, San Diego, CA, USA) diluted 1:50 for 16 h at 4 C
In rat models, transected Achilles tendons treated with BPC-157 showed faster functional recovery and denser, better-organised collagen at 2 and 4 weeks versus controls (Staresinic et al
How glutathione supplementation does helps in reducing oxidative stress
The peptide extends the anagen (growth) phase of hair follicles while shortening the telogen (resting) phase