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Comprehensive mechanistic validation across two-dimensional monolayer cultures and three-dimensional multicellular spheroid models demonstrated superior apoptotic induction, reactive oxygen species generation, nuclear fragmentation, cytoskeletal disruption, and migration suppression compared to single drug formulations, confirming that spatiotemporal codelivery enhances therapeutic synergy beyond additive effects

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Figure 2: Changes in the histolopathological scores amongst the four experimental treatment groups