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Consistently, the co-transfection assay of COP1-binding defective DET1 mutants including R488A/H485G, R488A/H485G/R306G/S312G and R488A/H485G/E380A/T382A with COP1 and Ube2e2 demonstrated that the reduction of COP1-DET1 interaction resulted in the decreased amount of Ube2e2 co-purification, suggesting that DET1-mediated proximity is not sufficient for COP1 and Ube2e2 to directly interact (Fig

The formula is simple: Milligrams delivered = (Units drawn 100) Concentration in mg/mL For 25 units: At 5 mg/mL: (25 100) 5 = 1.25 mg At 10 mg/mL: (25 100) 10 = 2.5 mg At 15 mg/mL: (25 100) 15 = 3.75 mg At 20 mg/mL: (25 100) 20 = 5 mg The same 25-unit draw delivers a four-fold difference in actual medication between the lowest and highest common concentrations

Segal M (2017) Dendritic spines: morphological building blocks of memory