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Compared to the wtVLP, our sVLP, which is derived from the PV1-SC7 mutant, carries seven amino acid substitutions: VP1 V196L, VP1 H248P, VP2 T25A, VP2 D57E, VP3 Q178L, VP3 L119M, and VP4 R18G (Fig
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The solubilized complex in the elution was immobilized by 2 h batch binding to M1 anti-Flag affinity resin at room temperature
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