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Effects of growth hormonereleasing hormone on cognitive function in adults with mild cognitive impairment and healthy older adults: Results of a controlled trial

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Insoluble immune complexes were rinsed once with Co-IP buffer then eluted by 0.1 mM citrate (pH3.0) twice for 5 min, resolved by sodium dodecyl sulfatepolyacrylamide gel, and transferred to nitrocellulose