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Following this, Fe 3+ is transported to the LIP via divalent metal-ion transporter-1 (DMT1) or ZRT/IRT-like proteins (ZIP), eventually being utilized by the organism after being reduced to Fe 2+ by the six-transmembrane epithelial antigen of prostate 3 (STEAP3) [25, 27]
10.1161/01.RES.84.11.1302 27
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This procedure was repeated with a second set of tubes run in parallel, but using assay buffer (provided in the total GLP-1 sandwich ELISA [EZGLP1T-36K, see later description]) instead of plasma
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