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Without a control group, it is impossible to determine whether the supplement contributed to these results or whether the lifestyle changes were responsible

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That choice addresses the symptom but not the mechanism and it introduces a new problem

Quantitative RT-PCR RNA was extracted from cell lysates using RNeasy Mini Kit (Qiagen, Chatsworth, CA, USA) and was reverse-transcribed using QuantiTect Reverse Transcription Kit (Qiagen, Chatsworth, CA, USA)