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The core equation is: Concentration (mg/mL) = peptide mass in the vial (mg) solvent volume added (mL) Once you know the concentration, the volume to inject for any desired dose is: Dose volume (mL) = desired dose (mg) concentration (mg/mL) Because insulin syringes are marked in units (100 IU = 1 mL on a U-100 syringe), it is often easiest to work in micrograms

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