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After a final wash step, a tetramethylbenzidine (TMB) peroxidase substrate solution (KPL Inc.) was added to each well and absorbance of the developed color was measured by a colorimetric plate reader (Molecular Device)

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It is also recommended to eat smaller, light, and bland meals, eat slowly, and avoid or limit alcohol consumption

Patients are encouraged to work with healthcare providers to develop a comprehensive plan that includes lifestyle modifications, ensuring a holistic approach to weight management and health improvement

Food Sci Technol