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Condensation of 6-formylpterin with the diester of p -aminobenzoyl-L-glutamic acid, followed by reduction of the Schiff base with sodium borohydride and hydrolysis, leads to folic acid 1,86,353,618

Flow cytometry analysis The cells from the cell sorting procedure were directly subject to flow cytometry analysis using NovoCyte Flow Cytometer (ACEA Bio)