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Using data from Real Chemistry's IRIS platformwhich consists of medical, hospital, and pharmacy claims as a primary data source, covering more than 300 million U.S

While not naturally occurring as an isolated fragment, its parent protein contributes to gastrointestinal protection and repair

Laboratory and animal studies suggest bile acids can activate the TGR5 receptor on L-cells, potentially promoting GLP-1 release

The cells were subsequently washed with 250 l lysis buffer, incubated for 15 min with 500 l lysis buffer, and incubated for 10 min in 500 l stimulation buffer (similar to lysis buffer but with 0 or 5 mmol/l ATP and 1.65 or 6.65 mmol/l CaCl 2 ) at 37 C, 5% CO 2