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To separate MCF derivatives from the reaction mixture, 400 L chloroform was added and vigorously mixed for 10 s followed by the addition of 400 L sodium bicarbonate (50 mM), and mixing for an additional 10 s
As PQQ is known to enhance mitochondrial function, it attenuated diet-induced fat accumulation primarily by improving beta-oxidation through mitochondrial biogenesis and suppressing lipogenesis (Figure 1B)
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