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Thyroid hormone, hypothyroidism, and mast cells: The thyroid is a small gland in the neck that produces hormones that regulate cell metabolism, cardiac output, respiratory rate, alertness, and reproductive health

For signal detection, the ABI Prism 7700 sequence detector (Life Technologies, CA, USA) was programmed to execute an initial step of 2 min at 50C and 10 min at 95C, followed by 40 thermal cycles of 15 sec at 95C and 1 min at 60C