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Cells were seeded at a density of 510 3 /well in 200 L of medium into 96-well plates (BIOFIL, Guangzhou, China), and treated with Erastin or ferrostatin-1 for the indicated time

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This interaction both disrupts peptidoglycan assembly in spore-forming bacilli and promotes the aggregation of nisinlipid complexes that create pores in the cytoplasmic membrane, leading to loss of membrane integrity and cell death (198, 199)

doi:10.1038/306284a0