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Pacing, which involves carefully managing physical and cognitive activity to avoid post-exertional crashes, is one of the most important self-management strategies
When trying to overcome this habit, the advice to just stop eating, isnt helpful because these neurological processes motivate someone to eat when uncomfortable feelings arise
For those concerned about potential discomfort, mild side effects like stomach pain can occur but are generally manageable
For signal detection, the ABI Prism 7700 sequence detector (Life Technologies, CA, USA) was programmed to execute an initial step of 2 min at 50C and 10 min at 95C, followed by 40 thermal cycles of 15 sec at 95C and 1 min at 60C
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