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Then cDNA was synthesized using SuperScript TM IV VILO TM Master Mix (Invitrogen, Waltham, MA, United States) and diluted 101 by DNase/RNase-Free Distilled Water (Invitrogen)

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Moreover, FOXO4-DRI was used to selectively induce apoptosis of senescent fibroblasts in both keloid fibroblast and organ culture senescent models, showing potential as a treatment that targets the senescence and apoptosis resistance observed in keloid fibroblasts