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After different groups of HUVECs are treated accordingly, they are seeded into 24-well plates pre-coated with 150 L/well growth factor-reduced Matrigel (Corning, catalog number 354234) and incubated at 37 C in a cell culture incubator for 12 h
Each vial is 0.5 ml, so the concentration is 2 mg/0.1 ml
Researchers may track changes in molecular markers, such as pathway readouts or gene-expression signals, to better understand how short peptides can probe age-related cellular processes in experimental models
There are, however, conflicting reports regarding the neuroinvasiveness of SARS-CoV-2 and indeed the cellular expression of the receptors 11,12,13,14,15 , suggesting that other mechanisms are responsible for the neurological problems reported
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