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The reality: Short-Term Illness Impact : A week of illness with reduced activity and altered eating patterns might cause temporary weight fluctuations, but these are primarily due to: Changes in hydration status Reduced physical activity Altered eating patterns Inflammation and fluid retention Long-Term Progress : Short illnesses dont significantly impact your overall weight trajectory
Wu YC, Wang WT, Lee SS, et al
When youre eating less, especially if youre not consuming as much red meat, your iron intake might drop
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Degradation assay Cells were treated in serum-free medium plus 11 mM glucose agonist (100 nM) before lysis (20 mM Tris base, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 0.5% Triton X-100 plus complete mini EDTA-free protease inhibitor (Roche)), addition of urea sample buffer (100 mM Tris-HCl pH 6.8, 2.5% SDS, 4 M urea, 100 mM DTT, 0.05% bromophenol blue), 10 min incubation at 37 C and immunoblotting
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