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EAAT2 prevents excitotoxicity by maintaining extracellular glutamate concentrations at ~25 nM [308, 370373]
The resulting concentration will be 10 mg per mL, which pairs well with a standard insulin syringe for easy measurement
If your levels are already normal, a shot mainly keeps you topped off rather than creating a big surge
Following treatment, cells were washed with PBS and then incubated with 500 L DCFH-DA working solution (20 M) at 37 C for 30 min
An exploration of their influence on macrophage and -cell crosstalk
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