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Membrane blocking was performed for 1 h with 5% skim milk in TBST at RT
This is the theoretical basis for endogenously supplied gamma-glutamylcysteines ability to raise GSH levels, as being the product of the GCL enzyme, it can be taken up by cells intact, where it feeds directly into glutathione synthase to be converted to glutathione, effectively bypassing the dysfunctional GCL
These findings support its inclusion in experimental longevity research discussions
These studies, using genetic manipulation and metabolite rescue experiments, confirm that the SSP acts as a driver of drug resistance
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