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2.8 ELISA tests for SIRT1, AMPK, p-AMPK, and Nrf2 levels Levels of SIRT1 (Cat# SL1254Ra), AMPK (Cat# SL1695Ra), p-AMPK (Cat# SL0570Ra), and Nrf2 (Cat# SL0985Ra) in kidney tissues were assessed using the ELISA method, following the manufacturers protocol (SunLong Biotech Co., Ltd., Hangzhou City, Zhejiang, China)

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Although the mean values for each of the alpha diversity measures were often largest for the 5A-1MQ-treated group switched from WD to LD relative to all other groups (Fig

In addition, oxidative stress contributes to a number of age-related illnesses, such as sarcopenia and frailty ( e.g ., cardiovascular diseases, obstructive pulmonary disease, diabetes, renal disease, neurodegenerative diseases, and cancer)