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PICRUSt [81] was conducted through an online application ( The relative abundance difference of bacteria and significantly different KEGG pathways within groups were estimated using STAMP v0.2.1.3 [82] and an extended error bar plot

The insights into the signaling pathway and transcription factor network of liver development provide cues for yielding hepatocyte-like cells (HLCs) from PSCs by chemical modulation

Cells were washed with PBS plus 0.02% Triton X-100 (Sigma-Aldrich, Dorset, UK) 3 times for 5 min each and blocked with 10% donkey serum (Sigma-Aldrich, Dorset, UK) for 30 min