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After fixation with 2% paraformaldehyde for 15 min, 4 C, blocking was performed with 3% BSA - 0.1% triton X-100 in PBS 1 (w/v/v) (AF938, R&D systems, Minneapolis, Minnesota, USA) for 30 min, room temperature

Maximum potency requirements favor injection

Gather supplies: have syringes, needles, alcohol pads, and a sharps container ready

Carrageenan-induced tendinopathy Injection of a family of carrageenans, linear sulfated polysaccharides that are extracted from red edible seaweeds, directly into a mouse tendon to establish tendinopathy

Clinical Monitoring of Oxidative Stress in Asthma Oxidants are produced at higher amounts either spontaneously or after the stimulation in patients with asthma compared with healthy subjects