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This improves glucose uptake in muscle cells and regulates glucose levels, which enhances insulin action and reduces markers of inflammation and oxidative stress, factors associated with diabetes

2.6 Gene expression RNA was extracted from frozen liver tissues using the RNeasy mini kit (Qiagen), treated with DNase I (Sigma-Aldrich), then reverse-transcribed with M-MLV reverse transcriptase (Promega)

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The same group adopted this method to identify inhibitors that target the BF3 191