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These EF-hand proteins are found both intracellularly and extracellularly

Real-time PCR of mRNA levels cDNA was synthesized from total RNA (1 g) extracted from mouse BAT using TRIzol Reagent (Invitrogen, Tokyo, Japan)

For such studies, bronchoconstriction, inflammation and lung function were primary endpoints

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The progressive drying and keratinization of the conjunctiva, particularly on the nasal and temporal conjunctiva, results in the development of irregularly shaped spots termed Bitot (bee toe) spots after the French physician, Pierre Bitot, who first described them