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That rules out, straightforwardly, radioligand or fluorescent binding assays, competition binding against a known ligand, recombinant receptor-expressing lines, functional readouts tied to a defined coupling pathway, and antagonist controls to demonstrate that an effect is receptor-mediated

One milliliter of culture was then back-diluted again into each of two flasks containing (1) 49 ml of DRM supplemented with 50 g ml 1 carbenicillin, 50 g ml 1 spectinomycin, 20 M arabinose and 20 g ml 1 chloramphenicol or (2) 49 ml of DRM supplemented with 50 g ml 1 carbenicillin, 50 g ml 1 spectinomycin and 20 M arabinose

This prevents the copper from binding correctly to the peptide, reducing its regenerative ability

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