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The percentage of hemolysis (Z) was calculated using the following formula: 2.6 Light microscopy Following treatment with ME and H 2 O 2 , 10 L of the erythrocyte suspension (3% hematocrit) was placed onto a microscope slide and gently covered with a coverslip to create an even monolayer, as previously described ( 2.7 Scanning electron microscopy For scanning electron microscopy (SEM), erythrocytes treated with DMSO or ME were washed with PBS and fixed in 2.5% glutaraldehyde for 1 h at room temperature with gentle shaking
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