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To verify the role of GSH in activating long-distance plant defense signaling events, we wounded leaf 1 of pad2-1 and Col-0, and then measured the accumulation of wound-induced plant defensive hormonal molecules in leaf 6, considering that leaf n shares direct vascular connections with leaves n 5 and n 8 while leaves n 3 may represent contact parastichies formed by proximal but unconnected vasculature in adult Arabidopsis rosettes 7,25
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