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Crucially, the researchers explored substituting these expensive growth factors with a small-molecule cocktail, specifically 10 g/mL Vitamin C, 0.1 M Dihexa, and 10 M Forskolin (VDF cocktail), to assess its efficacy in inducing hepatic specification
After 3 days, luciferase assays were performed as described above
Tang, S
Free Radic Biol Med 37:755767 Hirst J, King MS, Pryde KR (2008) The production of reactive oxygen species by complex I
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