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Nature 566 , 4950 (2019)

Sample preparation serum (lithium heparin) A volume of 25 l of serum were mixed with 250 l a cold solvent mixture with ISTD (methanol/water/chloroform, 9:1:1, 20 C), into 1.5 ml microtube, vortexed and centrifugated (10 min at 15,000 g , 4 C)