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2.9.3 RT-PCR using SYBR Green Primer testing was conducted using a 25-L reaction mix that contained 12.5 L of the 2x Quanti-Tect PCR SYBR Green Master Mix (Qiagen, Germany), 0.25 L of Revert-Aid Reverse Transcriptase (200 U/L) (Thermo Fisher), 0.5 L of each primer of 20 pmol, 8.25 L of water, and 3 L of RNA template
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At-risk groups, including the elderly, vegetarians, vegans, and individuals with gastrointestinal disorders, may need B12 injections for deficiency prevention
10.1021/tx9701687 20 ChengJ
The group said the term should be used in the context of drug substances, while degradation products would be used to refer to impurities formed in drug products
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