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They isolated the protein responsible, then synthesized the active 15-amino-acid fragment
These mechanisms involve different steps which are currently not well-characterized: (1) entrance of Ca 2+ into the enterocytes through the Ca 2+ -selective transient receptor potential (TRP) channel TRPV6 and the voltage-gated calcium channel Cav1.3, a member of the L-type calcium channel family
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Hydrochloric acid and/or sodium hydroxide may have been added during manufacture to adjust the pH (range 4.5-7.0)
Critical safety considerations for laboratory use include potential immunogenicity concernswith peptide therapeutics commonly showing injection site reactions (20-40% with subcutaneous administration) and possible antidrug antibody formationalong with theoretical concerns that pro-angiogenic properties could potentially influence experimental tumor models, requiring appropriate controls in oncology-related research
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