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Glutathione is synthesized in two sequential enzymatic reactions: glutamate cysteine ligase (GCL) catalyzes the first, rate-limiting step, and glutathione synthetase couples -glutamylcysteine to glycine in the last step [13]
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Iron accumulation drives the Fenton and Haber-Weiss reactions, generating ROS that induce lipid peroxidation, leading to oxidative damage to subcellular structures (88)
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