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Pellets were resuspended in 300 l PBS, and 10,000 events were analysed by flow cytometry using a BD Accuri C6 Plus flow cytometer (BD Biosciences) and FCS Express 7 Research (DeNovo Software)
Next, the GSH linked with the active site cysteine residue of OsDHAR (enzyme-S-S-G) is removed by nucleophilic attack of the H-site bound GS
PMCID: PMC4979965
Appropriate protective equipment such as gloves, lab coats, and safety glasses should always be worn when handling the substance
This may require months of careful management
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