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Supernatants of cells or tissues were stored at 80 C until analysis
This includes studies exploring how DSIP or DSIP-like compounds may influence processes such as oxidative stress (cellular damage caused by unstable molecules), neuronal signaling (communication between brain cells), seizure activity, adaptation to low-oxygen environments, and mitochondrial function, which helps regulate cellular energy production [1112][1819]
The GI side effects are dose-dependent
The upper solvent was evaporated under a stream of nitrogen, and the residue was dissolved in 150 L of methanol before LC-MS analysis
