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However, significant safety concerns and lack of regulatory approval limit their clinical utility
Monitoring milk output for signs of decreased production
For antibody labeling, after treatments at 37 C, cells were placed on ice to arrest further endocytosis before fixation, and the surface GLP-1R was detected by ELISA with monoclonal anti-human GLP-1R antibody 68 (Mab 3F52, Developmental Studies Hybridoma Bank (DSHB), 1/100) plus horseradish peroxidase (HRP)-conjugated rabbit anti-mouse secondary (ab97046, Abcam, 1/5,000)
It does show that the compounds biological activity was measurable at the tissue level in a controlled preclinical model