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After washes in PBS with 0.1% Tween (PBS-T) for 30 min (10 min per wash), a secondary antibody was applied for 1 h at room temperature in the blocking buffer (see Supplementary Table 4 for primary and secondary antibodies used in this study)
Laboratory research record on PT-141 Bremelanotide [PMID: 18206919] PubMed laboratory record discussing PT-141 Bremelanotide in a research context
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Recent developments in high-throughput NGS have enabled miRNA profiling in biofluids
