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Metab Brain Dis 25(4):381389
After 4 days, the medium was replaced with DMEM/F12 containing 3.3 mM biotin,1.7 mM pantothenic acid, 0.01 mg/ml transferrin, 20 nM insulin, 100 nM cortisol, 0.2 nM tri-iodothyronine every other day (Sigma-Aldrich)
In 1976, esteemed researcher Genant et al
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